Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Protective role of hydrogen sulfide against diabetic cardiomyopathy by inhibiting pyroptosis and myocardial fibrosis.

doi: 10.1016/j.biopha.2024.116613

Figure Lengend Snippet: Fig. 5. High glucose induced cardiomyocyte pyroptosis.A.Immunofluorescence was used to identify the purity of NRVMs.B.Calcein-AM/Hoechst 33342 staining was used to observe cardiomyocyte membrane injury caused by high glucose.C.Scanning electron microscopy was used to observe the effect of high glucose on NRVMs damage.D-G.High glucose induced increased expressions of pyroptosis related proteins in NRVMs.Values are expressed as mean±SD (n=4).*P<0.05,**P<0.01vs Control group.

Article Snippet: Initially, a 2 μM Calcein AM staining solution (Beyotime, China) was formulated using a serum-free cell culture medium.

Techniques: Immunofluorescence, Staining, Membrane, Electron Microscopy, Control

Journal: Stem Cell Research & Therapy

Article Title: Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration

doi: 10.1186/s13287-018-1032-9

Figure Lengend Snippet: Effects of IL-1β in morphology and interaction between HUVECs and MSCs. Labeled MSCs with CellTracker™ Orange were seeded on HUVECs stained with Calcein AM and co-cultivated for 30 to 240 min. After a period of 60 min, MSCs attached to HUVECs and the morphology were still spherical but developed form of cytoplasmic offshoot. a After 60 min, MSC became flattened and adhered to HUVEC monolayer. IL-1β promoted adhesion (left, 60 min) and transendothelial migration abilities (right, 240 min) of MSCs. b After 180 and 240 min, MSCs extended long plasmic filopodia and integrated into the HUVEC monolayer. Orthogonal projections illustrate that MSCs inserted into HUVEC monolayer (left, 180 min) and formation of filopodia caused transendothelial migration (right, 240 min). Arrows indicate MSC migration through the HUVEC. Horizontal bar: XZ plane of confocal image stack; vertical bar: YZ plane of confocal image stack. Scale bar = 10 μm

Article Snippet: HUVEC monolayers were stained with 8 μM Calcein AM (Tocris, UK) in serum-free medium for 30 min at 37 °C, then gently washed three times with PBS.

Techniques: Labeling, Staining, Migration

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Sauchinone preserves cardiac function in doxorubicin-induced cardiomyopathy by inhibiting the NLRP3 inflammasome.

doi: 10.1016/j.phymed.2025.156624

Figure Lengend Snippet: Fig. 2. Sauchinone (Sau) has potent cardioprotective effects on doxorubicin (Dox)-treated H9c2 cells. A, Chemical structural formula of Sau. B, Toxic effects of Sau on H9c2 cells were determined using the CCK-8 assay. n=5 per group. C, The effects of Sau on Dox- induced H9c2 cell viability. n=8 per group. D, LDH release from H9c2 cells. n=6 per group. E, Representative images of H9c2 cell morphology. F, The ratio of dead cells to live cells was analyzed based on the images in E. n=5 per group. G, Representative images of Calcein-AM & PI staining, along with the ratio of dead cells to live cells in H9c2 cells. n=8 per group. H, ATP production in H9c2 cells. n=8 per group. I, The expression levels of BAX and BCL2 proteins in H9c2 cells. n=4 per group for BAX and n=5 per group for BCL2. J, The levels of Bax and Bcl2 mRNA in H9c2 cells. n=3 per group. K, The expression of BAX and BCL2 protein in left ventricle tissues. n=4 per group. L, The levels of Bax and Bcl2 mRNA in left ventricle tissues. n=4 per group. Data are presented as mean±SEM. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. #, crystal precipitation of Sau. One-way ANOVA was used in B. Two-way ANOVA was used in C-L.

Article Snippet: Calcein AM and propidium iodide (PI) staining Calcein AM and PI double staining was conducted to further evaluate cell death using a commercially available kit from Elabscience® (E-CKA354, Wuhan, China).

Techniques: CCK-8 Assay, Staining, Expressing

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Sauchinone preserves cardiac function in doxorubicin-induced cardiomyopathy by inhibiting the NLRP3 inflammasome.

doi: 10.1016/j.phymed.2025.156624

Figure Lengend Snippet: Fig. 5. Sauchinone (Sau) inactivates the NLRP3 inflammasome to suppress doxorubicin (Dox)-induced cardiomyocyte apoptosis and oxidative injury. A, The expression levels of NLRP3, ASC, and Caspase1 in H9c2 cells. n=6 for NLRP3 and n=4 for ASC or Caspase1. B, The expression of NLRP3, ASC, and Caspase1 in cardiac tissues. n=3 per group. C, The effects of Sau and MCC950 or Nigericin (Niger) on the cell viability of Dox-treated H9c2 cells. n=6 per group. D, LDH release from H9c2 cells. n=6 per group. E, ATP production in H9c2 cells. n=6 per group. F, The mRNA levels of Bax and Bcl2 in H9c2 cells. n=3 per group. G-H, Representative images of Calcein-AM & PI staining, along with the ratio of dead cells to live cells in H9c2 cells. n=6 per group. I-J, The mitochondrial membrane potential was assessed using the JC-1 fluorescent probe. K-L, The levels of reactive oxygen species (ROS) were analyzed using the DCFH-DA probe. n=6 per group. Data are presented as mean±SEM. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. Two-way ANOVA was used.

Article Snippet: Calcein AM and propidium iodide (PI) staining Calcein AM and PI double staining was conducted to further evaluate cell death using a commercially available kit from Elabscience® (E-CKA354, Wuhan, China).

Techniques: Expressing, Staining, Membrane